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The Australian Customer’s V947 Autosampler Vial Order is Completed
The Australian Customer’s V947 Autosampler Vial Order is Completed
American Customer Ordered 100,000 Boxes of 9-425 2ml HPLC Vials
American Customer Ordered 100,000 Boxes of 9-425 2ml HPLC Vials
5000-boxes-of-blue-screw-cap-10-425-hplc-vial-sent-to-singapore
5000-boxes-of-blue-screw-cap-10-425-hplc-vial-sent-to-singapore
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SW-846 does not name “HPLC vial” but 8000C and the method-specific chapters
SW-846 does not name “HPLC vial” but 8000C and the method-specific chapters
April 5, 2018
SW-846 does not name “HPLC vial” but 8000C and the method-specific chapters
A 24-sample semivolatiles run just failed its continuing calibration verification because the internal standard response in vial 7 dropped 41% from vial 1. The method was EPA 8270E. The instrument checked out, the column was fine, and the standard was fresh. The problem was the vial: a lot of 9 mm screw caps with PTFE/silicone septa that had been autoclaved once too often and then torqued to 18 in-lbs by a new technician. The septa had compressed unevenly, the vial leaked during the 12-hour...
Seal failure is a mass transfer problem, not a leak in the plumbing sense
Seal failure is a mass transfer problem, not a leak in the plumbing sense
April 5, 2018
Seal failure is a mass transfer problem, not a leak in the plumbing sense
A 96-vial overnight run on a stability assay came back with area counts 14% high on the last 20 injections. The mobile phase was fine, the column was fine, the autosampler needle wasn’t clogged. The problem was evaporation through the septa. Every vial lost between 18 and 31 mg of solvent over 11 hours. That weight loss concentrated the analytes, and the calibration curve no longer matched the samples. If you run long sequences or store prepared samples before injection, hplc vial sea...
Tested values carry a method, nominal values carry a tolerance that may not
Tested values carry a method, nominal values carry a tolerance that may not
April 5, 2018
Tested values carry a method, nominal values carry a tolerance that may not
A purchasing agent forwarded me a datasheet last month for a 9 mm screw-top vial. It listed “Residual volume: < 5 µL" and "Septum resealability: > 50 injections.” Both numbers looked fine on paper. The first value was never tested on that lot. The second was tested on a different septum, from a different production year, against a 26-gauge conical needle at 23 °C. The agent had already placed a PO. Reading an HPLC vial datasheet is not about finding the best numbers. It is about...